Coding

Part:BBa_K2706001:Design

Designed by: Elizabeth Chamiec-Case   Group: iGEM18_UConn   (2018-10-09)


tesA (L109P) with pTac, rbs (BBa_B0034), and terminator (BBa_B0015)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 543
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 148
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

synthesized with a mutation to change amino acid 109 leucine to proline


Source

'tesA naturally occurs in E. coli but was mutated for the purposes of increased output

References